A study by Machado et al. analysed the genetic diversity of four cattle breeds using microsatellite markers. The results showed that the Holstein breed was the most distinct from the other breeds. A total of 64 alleles were detected across all four breeds, with each breed showing 53% of the total number of alleles. The average number of alleles per locus was 7.11 ± 3.21. The study provides valuable insight into the genetic diversity of different cattle breeds and has implications for breeding decisions and the preservation of genetic resources.